jEnesisDS does not make use of the RAM pack. SNEmulDS can use it for large SNES games (though I haven't really noticed any difference) while some other programs can use it as well, most notably Quake DS, Quake 2 DS, NeoDS, DSLinux, and Moonshell (for decoding large JPEG images). Those are the only ones I can think of that use slot-2 RAM at all.
Note that GBA files cannot be run on the Opera pack, so if you're going to get slot-2 RAM you'd be better off with something like the EZ-V 3in1 expansion pack.
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